bladder transitional cell carcinoma  (DSMZ)

Journal: Cancers

Article Title: Inducing Targeted, Caspase-Independent Apoptosis with New Chimeric Proteins for Treatment of Solid Cancers.

doi: 10.3390/cancers17071179

Figure Lengend Snippet: Figure 4. GnRH-AIF chimeric protein kills target cancer cells in a dose- and time-dependent manner. Colo205 and LNCaP cells were treated with various doses of GnRH-AIF and GnRH-AIFinact (0.5, 0.7, and 1 µM) and incubated for 24, 48, or 72 h demonstrating a dose- and time-dependent cell death (A,B). Simultaneously, Colo205, LNCaP, MCF-7, SW48, Colo320, HEK293, and A549 were treated with GnRH-AIF (1 µM) and GnRH-AIFinact mutant chimera (1 µM) for 72 h and tested under the same conditions (C). A204 cells, normal fibroblasts, and T24P cancer cells lacking GnRH-R expression were treated with GnRH-AIF or PBS (D). Results (A–D) are presented as percentage of cell death calculated for treated cells as compared to control cells (treated with PBS only). All results are the average of 3–6 repeats of the experiments ± SD. p value ≤0.01 = **; p value ≤0.001 = ***; inact = inactive.

Article Snippet: A204 rhabdomyosarcoma cells (ATCC-HTB-82), T24P bladder urinary transitional cell carcinoma (ATCC-HTB-4), normal fibroblasts, (ATCC-PCS-420-013), lung A549 cells (ATCC-CCL-185), and HEK-293 renal adenocarcinoma cells (ATCC-CRL-1573) were grown in DMEM medium (01-055-1A, Sartorius) supplemented with 10% FBS, 2 mM L-glutamine, and 100 units/mL penicillin.

Techniques: Incubation, Mutagenesis, Expressing, Control